Maeda M, Mizuki E, Nakamura Y, Hatano T, Ohba M
Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka 803-0814, Japan.
Curr Microbiol. 2000 Jun;40(6):418-22. doi: 10.1007/s002840010080.
Marine sediments from a Japanese bay were examined for the occurrence of Bacillus thuringiensis. Of 1313 colonies belonging to the Bacillus cereus/B. thuringiensis group, 22 (1.7%) were allocated to B. thuringiensis. Marine isolates of B. thuringiensis consisted of heterogeneous multiple H serogroups; 10 isolates were assigned to the eight serovars (kurstaki, sumiyoshiensis, sotto, aizawai, darmstadiensis, thompsoni, neoleonensis, and higo); two motile isolates failed to react with the reference antisera; and the others were serologically untestable. Insecticidal activities were associated with two kurstaki isolates (toxic to both Lepidoptera and Diptera) and a higo isolate (Diptera-specific). None of the parasporal inclusion proteins of the 22 isolates exhibited in vitro cytotoxic activity against two vertebrate cells, sheep erythrocytes and HeLa cells. All B. thuringiensis isolates had no halophilism, although seawater-based medium supported their growth, sporulation, and formation of parasporal inclusions.
对取自日本一个海湾的海洋沉积物进行了苏云金芽孢杆菌的检测。在属于蜡样芽孢杆菌/苏云金芽孢杆菌组的1313个菌落中，有22个（1.7%）被鉴定为苏云金芽孢杆菌。苏云金芽孢杆菌的海洋分离株由多种不同的H血清群组成；10个分离株被归入8个血清型（库尔斯塔克型、住吉型、 sotto型、阿瓦亚种、达姆施塔迪型、汤普森型、新莱昂型和日向型）；2个运动性分离株与参考抗血清无反应；其他分离株无法进行血清学检测。杀虫活性与2个库尔斯塔克型分离株（对鳞翅目和双翅目均有毒性）和1个日向型分离株（对双翅目有特异性毒性）有关。22个分离株的伴孢晶体蛋白均未对两种脊椎动物细胞、绵羊红细胞和HeLa细胞表现出体外细胞毒性活性。所有苏云金芽孢杆菌分离株均无嗜盐性，尽管基于海水的培养基支持它们的生长、孢子形成和伴孢晶体的形成。
