Tianjin University of Science and Technology, China.
Appl Biochem Biotechnol. 2010 Jan;160(2):532-8. doi: 10.1007/s12010-008-8412-z. Epub 2008 Nov 12.
Ethanol productions from cheese whey powder (CWP) solution were investigated by using free or immobilized cells of Kluyveromyces marxianus in monocultures or mixed cultures with free or immobilized cells of K. marxianus and Saccharomyces cerevisiae. K. marxianus free cells produced 3.8% v/v ethanol in monocultures, while S. cerevisiae immobilized cells produced 5.3% v/v ethanol in mixed cultures. The percentage of theoretical yield was found to be higher in mixed cultures than that in monocultures. The maximum ethanol fermentation efficiency was achieved (79.9% of the theoretical value) using mixed cultures of immobilized cells of K. marxianus and S. cerevisiae. The beads were relatively stable without significant reduction in activity for about eight batches of fermentation.
利用马克斯克鲁维酵母游离细胞或固定化细胞、马克斯克鲁维酵母游离细胞或固定化细胞与酿酒酵母固定化细胞的混合培养物，从奶酪乳清粉（CWP）溶液中生产乙醇。马克斯克鲁维酵母游离细胞在纯培养中产生 3.8%v/v 的乙醇，而酿酒酵母固定化细胞在混合培养中产生 5.3%v/v 的乙醇。发现混合培养中的理论产率百分比高于纯培养中的理论产率百分比。使用马克斯克鲁维酵母固定化细胞和酿酒酵母固定化细胞的混合培养物，可实现最大的乙醇发酵效率（达到理论值的 79.9%）。这些珠粒相对稳定，在大约 8 批发酵过程中没有明显的活性下降。
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