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通过流式细胞术从各种小鼠组织中简单快速地回收细胞并进行 DNA 含量分析的方法。

A simple and fast method for cell recovery and DNA content analysis from various mouse tissues by flow cytometry.

机构信息

Inserm U781, Clinique M. Lamy, Hôpital Necker Enfants Malades, 149 rue de Sèvres, 75015, Paris, France.

出版信息

Cytotechnology. 2006 Oct;52(2):107-12. doi: 10.1007/s10616-006-9036-1. Epub 2006 Dec 14.

DOI:10.1007/s10616-006-9036-1
PMID:19002869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449417/
Abstract

Cell division in tissues can be investigated in various ways. We present here a method for improving cell recovery and cell cycle analysis for a wide range of mouse tissues. This strategy combines a cell isolation procedure for various mouse tissues based on intracardiac perfusion and subsequent treatment followed by flow cytometry. This easy and reproducible method allows a rapid analysis of nuclear DNA content, providing an estimate of the cell number at different phases of the cell cycle. This combined procedure could also be used for the isolation of specific cell subpopulations from different mouse tissues by fluorescence activated cell sorting.

摘要

组织中的细胞分裂可以通过多种方式进行研究。我们在这里介绍了一种用于提高多种小鼠组织中细胞回收和细胞周期分析效率的方法。该策略结合了基于心脏内灌注的各种小鼠组织的细胞分离程序以及随后的流式细胞术处理。这种简单且可重复的方法可以快速分析核 DNA 含量，估计细胞周期各阶段的细胞数量。这种联合程序也可用于通过荧光激活细胞分选从不同的小鼠组织中分离特定的细胞亚群。

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本文引用的文献

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Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice.给雄性大鼠和小鼠注射氚标记胸腺嘧啶核苷后，通过放射自显影术显示的细胞增殖和迁移情况。
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