通过碘化丙啶染色对哺乳动物细胞周期进行快速流式细胞荧光分析。
Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.
作者信息
Krishan A
出版信息
J Cell Biol. 1975 Jul;66(1):188-93. doi: 10.1083/jcb.66.1.188.
Abstract
A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.
摘要
本文描述了一种用于流式细胞荧光测定法快速测定每个细胞DNA含量的方法。将细胞置于碘化丙啶的低渗溶液中孵育，会导致细胞膜破裂，细胞核染色质快速染色。用该方法染色的细胞生成的DNA分布直方图与固定和核糖核酸酶消化后生成的直方图相同。与一些早期描述的方法相比，本技术快速（处理时间5分钟），所需材料最少，且避免细胞团块的形成。
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A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性
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The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.在用于分离闭环DNA的染料浮力密度法中使用溴化乙锭类似物：线粒体DNA超螺旋密度的变化
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Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations.对来自大型哺乳动物细胞群体的单细胞中的DNA、蛋白质和细胞体积进行快速、同步测量。
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