利用蓝藻气室作为细胞培养中的氧载体。
Use of cyanobacterial gas vesicles as oxygen carriers in cell culture.
机构信息
Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325-3906, USA.
出版信息
Cytotechnology. 2006 Oct;52(2):139-49. doi: 10.1007/s10616-007-9044-9. Epub 2007 Feb 20.
Abstract
The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture. Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco's Modified Eagle's Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen carrying capacity, can be used to increase the oxygen supply in cell culture systems.
摘要
从丝状蓝藻鱼腥藻细胞中分离出的气室用戊二醛处理和灭菌，然后评估其作为细胞培养中气载体的效果。依赖附着的非洲绿猴肾细胞（Vero）在微载体珠的填充床中生长，在杜氏改良伊格尔培养基（含 1%血清）的灌注下进行培养。结果发现，添加 1.8%（体积/体积）气室的培养基比不含气室的相同培养基支持更高的最大葡萄糖利用率，高出 30%。在 T 瓶培养中，气室悬浮液对细胞代谢没有明显影响。研究结果表明，具有高携氧能力的气室可用于增加细胞培养系统中的氧气供应。
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