在肽脂膜上培养的 NB1-RGB 细胞产生干扰素-β。

Production of interferon-beta by NB1-RGB cells cultured on peptide-lipid membranes.

机构信息

Department of Applied Chemistry, Seikei University, 3-1 Kichijoji Kitamachi 3, Musashino, 180-8633, Japan.

出版信息

Cytotechnology. 2003 May;42(1):13-20. doi: 10.1023/A:1026157515032.

DOI:10.1023/A:1026157515032

PMID:19002924

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449508/

Abstract

Cell growth and production of interferon-beta (IFN-beta) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and arginine-glutamic acid-aspartic acid-valine [Arg-Glu-Asp-Val] (REDV) peptides. Cell density was found to be approximately the same on various peptide-lipid membranes, whereas production of IFN-beta depended significantly on the peptide-lipid membranes on which NB1-RGB cells were cultured. The highest production of IFN-beta was observed for NB1-RGB cells on REDV-lipid membranes prepared by a casting method (REDV-cast membranes) after 24 hr of cultivation. Specific binding between REDV of REDV-cast membranes and the receptor on the NB1-RGB cells may have caused the specific cell response for the production of IFN-beta.

摘要

研究了在含有精氨酸-甘氨酸-天冬氨酸[Arg-Gly-Asp]（RGD）、酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸[Tyr-Ile-Gly-Ser-Arg]（YIGSR）和精氨酸-谷氨酸-天冬氨酸-缬氨酸[Arg-Glu-Asp-Val]（REDV）肽的肽脂质制备的膜上培养的正常人类皮肤成纤维细胞（NB1-RGB）的细胞生长和干扰素-β（IFN-β）的产生。发现细胞密度在各种肽脂质膜上大致相同，而 NB1-RGB 细胞培养的肽脂质膜对 IFN-β的产生有显著影响。培养 24 小时后，在用浇铸法制备的 REDV 脂质膜（REDV-cast 膜）上，NB1-RGB 细胞产生的 IFN-β 最多。REDV-cast 膜上的 REDV 与 NB1-RGB 细胞上的受体之间的特异性结合可能导致了产生 IFN-β 的特异性细胞反应。