Nuttelman C R, Mortisen D J, Henry S M, Anseth K S
Department of Chemical Engineering, University of Colorado, Boulder, 80309-0424, USA.
J Biomed Mater Res. 2001 Nov;57(2):217-23. doi: 10.1002/1097-4636(200111)57:2<217::aid-jbm1161>3.0.co;2-i.
Hydrogels have been used in biology and medicine for many years, and they possess many properties that make them advantageous for tissue engineering applications. Their high water content and tissue-like elasticity are similar to the native extracellular matrix of many tissues. In this work, we investigated the potential of a modified poly(vinyl alcohol) (PVA) hydrogel as a biomaterial for tissue engineering applications. First, the ability of NIH3T3 fibroblast cells to attach to PVA hydrogels was evaluated. Because of PVA's extremely hydrophilic nature, important cell adhesion proteins do not adsorb to PVA hydrogels, and consequently, cells are unable to adhere to the hydrogel. By covalently attaching the important cell adhesion protein fibronectin onto the PVA hydrogel surface, the rate of fibroblast attachment and proliferation was dramatically improved, and promoted two-dimensional cell migration. These studies illustrate that a fibronectin-modified PVA hydrogel is a potential biomaterial for tissue engineering applications.
水凝胶在生物学和医学领域已应用多年，它们具有许多特性，使其在组织工程应用中具有优势。其高含水量和类似组织的弹性与许多组织的天然细胞外基质相似。在这项工作中，我们研究了一种改性聚乙烯醇（PVA）水凝胶作为组织工程应用生物材料的潜力。首先，评估了NIH3T3成纤维细胞附着于PVA水凝胶的能力。由于PVA具有极强的亲水性，重要的细胞粘附蛋白不会吸附到PVA水凝胶上，因此细胞无法粘附到水凝胶上。通过将重要的细胞粘附蛋白纤连蛋白共价连接到PVA水凝胶表面，成纤维细胞的附着和增殖速率显著提高，并促进了二维细胞迁移。这些研究表明，纤连蛋白改性的PVA水凝胶是一种潜在的组织工程应用生物材料。
