Department of Developmental Biology, The University of Plovdiv, 24, Tzar Assen Str., 4000 Plovdiv, Bulgaria(e-mail,
Cytotechnology. 2003 Sep;42(3):163-7. doi: 10.1023/B:CYTO.0000015846.81124.3b.
In this paper, we offer detailed information concerning manipulations with the novel serum-free cell line McCoy-Plovdiv. Guidelines for procedures as trypsinization of the monolayer, subculturing, as well as freezing and thawing conditions are proposed. Our results give us grounds to assume that this is a cell line entirely serum-independent at any step of the process of culturing and preservation. The serum-free cell line McCoy-Plovdiv enriches the collection of mammalian serum-free cell lines. The easier cultivation, the lower expenses and the higher sensitivity in comparison with the serum-supplemented McCoy cells are discussed as possibilities for broad applications of McCoy-Plovdiv cells in different types of laboratory investigations.
在本文中，我们提供了有关新型无血清细胞系 McCoy-Plovdiv 操作的详细信息。提出了用于单层胰蛋白酶消化、传代、以及冷冻和解冻条件的操作指南。我们的结果使我们有理由假设，在培养和保存过程的任何步骤中，这都是一种完全不需要血清的细胞系。无血清细胞系 McCoy-Plovdiv 丰富了哺乳动物无血清细胞系的集合。讨论了无血清细胞系 McCoy-Plovdiv 的更容易培养、更低成本和更高敏感性，作为其在不同类型实验室研究中广泛应用的可能性。
