Nogueira Y L
Serviço de Virologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz. 1998 Jul-Aug;93(4):509-14. doi: 10.1590/s0074-02761998000400017.
To demonstrate the potential of McCoy cells for the isolation of rabies virus from the cerebrospinal (CSF) fluid of a patient with a diagnosis of rabies, McCoy cells were inoculated with CSF from a patient with a clinical diagnosis of rabies and investigated in terms of morphometric aspect using the JAVA analysis system for the quantification of the increased size of infected cells compared to noninfected cells. The cells were also examined in terms of specific staining for the diagnosis of rabies by the method of Sellers for the observation of intracytoplasmic inclusions and by specific immunofluorescence staining for rabies virus. Infected cells showed changes in cell permeability and morphologic modifications which differed significantly compared to normal cells (P < 0.001) when analyzed by the Mann-Whitney and Kruskal-Wallis tests. Intense activity of the endoplasmic reticulum was also observed, as indicated by the presence of intracytoplasmic inclusions visualized by specific staining. The present study demonstrated the isolation of rabies virus from the CSF of a patient with rabies, showing that McCoy cells can be used for the laboratory diagnosis of patients suspected to have rabies.
为了证明 McCoy 细胞从临床诊断为狂犬病患者的脑脊液（CSF）中分离狂犬病病毒的潜力，将 McCoy 细胞接种来自临床诊断为狂犬病患者的脑脊液，并使用 JAVA 分析系统从形态计量学方面进行研究，以量化与未感染细胞相比受感染细胞增大的尺寸。还通过 Sellers 方法观察胞浆内包涵体对细胞进行狂犬病诊断的特异性染色，并对狂犬病病毒进行特异性免疫荧光染色检查细胞。通过 Mann-Whitney 和 Kruskal-Wallis 检验分析时，受感染细胞与正常细胞相比显示出细胞通透性变化和形态学改变，差异有统计学意义（P < 0.001）。通过特异性染色观察到胞浆内包涵体的存在，这也表明内质网有强烈活动。本研究证明从狂犬病患者的脑脊液中分离出了狂犬病病毒，表明 McCoy 细胞可用于疑似狂犬病患者的实验室诊断。
