用 Ni2+ 或 Co2+ 盐提高台盼蓝排斥试验的灵敏度。
Improved sensitivity of trypan blue dye exclusion assay with Ni2+ or Co2+ salts.
机构信息
Department of Organic Chemistry and Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India.
出版信息
Cytotechnology. 2000 Feb;32(2):93-5. doi: 10.1023/A:1008144527206.
Abstract
A modified trypan blue dye exclusion assay was developed usingNi(2+) or Co(2+) salts to determine the viability ofprimary and transformed cells. When the cells were preincubatedwith NiSO(4) or CoCl(2) followed by trypan blue assay, thecontrast between stained and unstained cells was significantlyincreased as compared to the conventional trypan blue dyeexclusion assay.
摘要
采用 Ni(2+)或 Co(2+)盐改良台盼蓝排斥试验检测原代和转化细胞的活力。与传统的台盼蓝排斥试验相比，细胞先用 NiSO(4)或 CoCl(2)预孵育，然后用台盼蓝染色，着色细胞与不着色细胞的对比度显著增加。
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