细胞活力的台盼蓝排斥试验
Trypan Blue Exclusion Test of Cell Viability.
作者信息
Strober Warren
机构信息
National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland.
出版信息
Curr Protoc Immunol. 2015 Nov 2;111:A3.B.1-A3.B.3. doi: 10.1002/0471142735.ima03bs111.
Abstract
The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).
摘要
本附录中描述的方案可用于通过光学显微镜对细胞活力进行定量分析。将细胞悬浮于含有台盼蓝的磷酸盐缓冲液（PBS）中，然后进行检查，以确定细胞质清亮的细胞（活细胞）与细胞质呈蓝色的细胞（死细胞）的百分比。
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