bFGF、IGF-I 和 IGF-II 表达或共表达的 3T3 细胞在低血清和无血清培养基中的存活。
Survival of 3T3 cells expressing or co-expressing bFGF and/or IGF-I and/or IGF-II in low serum and serum free media.
机构信息
Department of Biological Sciences, University of Paisley, Paisley, PA1 2BE, U.K..
出版信息
Cytotechnology. 2000 Mar;32(3):209-18. doi: 10.1023/A:1008151302358.
The aim of this study was to improve the survival ofNIH3T3 cells in low serum or serum-free media by theendogenous expression of recombinant bFGF (basicfibroblast growth factor) and/or IGF (insulin-likegrowth factor) -I and II. Expression was detected byWestern blotting, and the growth characteristics ofdifferent transfected cell lines investigated in bothserum-free and low serum conditions and also in softagar. Morphological changes and the growth rate werecompared with growth in normal serum-containingmedium. The experimental data suggested that theexpression of either bFGF alone, or the co-expressionof bFGF and either IGF-I or II could improve thesurvival of NIH3T3 cells in low serum or serum-freemedia. The use of such lines could decrease the use ofserum in cell culture and thus both reduce the costsinvolved in this technique and simplify thedown-stream purification procedure in protein harvest.Hence, such lines may be of value in both experimentaland industrial applications.
本研究旨在通过内源性表达重组 bFGF（碱性成纤维细胞生长因子）和/或 IGF（胰岛素样生长因子）-I 和 II 来提高 NIH3T3 细胞在低血清或无血清培养基中的存活率。通过 Western blot 检测表达情况，并在无血清和低血清条件下以及软琼脂中研究不同转染细胞系的生长特性。将形态变化和生长速率与正常含血清培养基中的生长进行比较。实验数据表明，单独表达 bFGF，或同时表达 bFGF 和 IGF-I 或 II 均可提高 NIH3T3 细胞在低血清或无血清培养基中的存活率。使用这种细胞系可以减少细胞培养中血清的使用，从而降低该技术的成本，并简化蛋白质收获时的下游纯化步骤。因此，这些细胞系在实验和工业应用中可能具有价值。
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