Advanced Research Institute for Science and Engineering, Waseda University, Higashi-Fushimi, Nishitokyo, 202-0021, Japan.
Cytotechnology. 2005 Sep;49(1):67-70. doi: 10.1007/s10616-005-5414-3.
Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.
制备核提取物是生化鉴定在核内发挥作用的因子（如转录因子）的关键步骤。我们已经建立了一种从黑腹果蝇的预蛹或蛹中制备核提取物的新方法。该方法简单，特别适用于从小量材料中分离，这些材料难以大量获得，如特定组织或被蛹壳覆盖的动物体。
