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家蚕FTZ-F1同源物的鉴定与纯化。

Identification and purification of a Bombyx mori homologue of FTZ-F1.

作者信息

Ueda H, Hirose S

机构信息

Genetic Stock Center, National Institute of Genetics, Mishima, Japan.

出版信息

Nucleic Acids Res. 1990 Dec 25;18(24):7229-34. doi: 10.1093/nar/18.24.7229.

DOI:10.1093/nar/18.24.7229
PMID:2124348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC332857/
Abstract

Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophila melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B. mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.

摘要

从家蚕胚胎以及后部和中部丝腺中提取的物质含有一种序列特异性DNA结合因子，称为BmFTZ-F1。该因子与FTZ-F1的识别位点结合，FTZ-F1是黑腹果蝇中fushi tarazu基因的一个正向调节因子。BmFTZ-F1和FTZ-F1具有相同的甲基化干扰模式、相同的色谱行为和相似的蛋白酶消化图谱。抗FTZ-F1与BmFTZ-F1发生交叉反应。这些结果表明BmFTZ-F1是FTZ-F1在家蚕中的同源物。丝腺提取物中形成的因子-DNA复合物的迁移率随发育阶段而变化。将BmFTZF1纯化至几乎均一的状态表明该因子是一种73kd的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/a062d75c3576/nar00208-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/4d0a69a090e2/nar00208-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/019c4962bd22/nar00208-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/b4ebcaebfa43/nar00208-0028-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/7610643fdd67/nar00208-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/a062d75c3576/nar00208-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/4d0a69a090e2/nar00208-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/019c4962bd22/nar00208-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/b4ebcaebfa43/nar00208-0028-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/7610643fdd67/nar00208-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6398/332857/a062d75c3576/nar00208-0030-a.jpg

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本文引用的文献

1
Repeated turn-off and turn-on of fibroin gene transcription during silk gland development of Bombyx mori.家蚕丝腺发育过程中丝素基因转录的反复开启和关闭。
Dev Biol. 1980 Aug;78(2):394-406. doi: 10.1016/0012-1606(80)90343-7.
2
Faithful transcription initiation of fibroin gene in a homologous cell-free system reveals an enhancing effect of 5' flanking sequence far upstream.在同源无细胞系统中丝心蛋白基因的忠实转录起始揭示了远上游5'侧翼序列的增强作用。
Cell. 1981 Nov;27(1 Pt 2):175-82. doi: 10.1016/0092-8674(81)90371-8.
3
Analysis of larval segmentation in lethal genotypes associated with the antennapedia gene complex in Drosophila melanogaster.
利用Illumina Hiseq 2000对日本沼虾雄性腺进行转录组分析以发现新基因
PLoS One. 2013 Oct 28;8(10):e76840. doi: 10.1371/journal.pone.0076840. eCollection 2013.
4
Ecdysone-responsive transcription factors determine the expression region of target cuticular protein genes in the epidermis of Bombyx mori.蜕皮激素反应转录因子决定了家蚕表皮中靶标表皮蛋白基因的表达区域。
Dev Genes Evol. 2012 Apr;222(2):89-97. doi: 10.1007/s00427-012-0392-x. Epub 2012 Mar 31.
5
Molecular cloning and expression analysis of fushi tarazu factor 1 in the brain of air-breathing catfish, Clarias gariepinus.气呼吸鲶鱼脑内 fushi tarazu factor 1 的分子克隆与表达分析。
PLoS One. 2011;6(12):e28867. doi: 10.1371/journal.pone.0028867. Epub 2011 Dec 28.
6
A Simple and Quick Method to Isolate Nuclear Extracts from Pupae of Drosophila melanogaster.一种简单快速的方法从黑腹果蝇蛹中分离核提取物。
Cytotechnology. 2005 Sep;49(1):67-70. doi: 10.1007/s10616-005-5414-3.
7
Drosophila Blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway.果蝇B淋巴母细胞诱导成熟蛋白-1是一种瞬时转录抑制因子，可控制蜕皮激素诱导的发育途径的时间。
Mol Cell Biol. 2007 Dec;27(24):8739-47. doi: 10.1128/MCB.01304-07. Epub 2007 Oct 8.
8
Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein.多蛋白桥接因子1（MBF1）是一种进化上保守的转录共激活因子，它连接调节因子和TATA元件结合蛋白。
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9
Regulation of the EDG84A gene by FTZ-F1 during metamorphosis in Drosophila melanogaster.果蝇变态发育过程中FTZ-F1对EDG84A基因的调控。
Mol Cell Biol. 1996 Nov;16(11):6509-15. doi: 10.1128/MCB.16.11.6509.
10
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Mol Cell Biol. 1994 May;14(5):3013-21. doi: 10.1128/mcb.14.5.3013-3021.1994.
黑腹果蝇中与触角足基因复合体相关的致死基因型幼虫体节分析。
Dev Biol. 1981 Jan 15;81(1):51-64. doi: 10.1016/0012-1606(81)90347-x.
4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Purification and properties of Drosophila heat shock activator protein.果蝇热休克激活蛋白的纯化及特性
Science. 1987 Nov 27;238(4831):1247-53. doi: 10.1126/science.3685975.
6
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Proc Natl Acad Sci U S A. 1986 Dec;83(24):9522-6. doi: 10.1073/pnas.83.24.9522.
7
Temporal and spatial control of silk gene transcription analyzed by nuclear run-on assays.通过核转录分析对丝基因转录的时空控制。
Dev Biol. 1988 Jun;127(2):384-91. doi: 10.1016/0012-1606(88)90325-9.
8
Interaction of composite protein complex with the fibroin enhancer sequence.复合蛋白复合物与丝心蛋白增强子序列的相互作用。
J Biol Chem. 1988 Apr 25;263(12):5979-86.
9
Expression, modification, and localization of the fushi tarazu protein in Drosophila embryos.果蝇胚胎中无尾蛋白的表达、修饰及定位
Genes Dev. 1988 Aug;2(8):1021-36. doi: 10.1101/gad.2.8.1021.
10
Tissue-specific and periodic changes in the nuclease sensitivity of the fibroin gene chromatin in the silkworm Bombyx mori.家蚕中丝素基因染色质核酸酶敏感性的组织特异性和周期性变化。
J Biol Chem. 1987 Apr 15;262(11):5271-9.