用于基因治疗目的的病毒载体生产的细胞培养工艺。
Cell culture processes for the production of viral vectors for gene therapy purposes.
机构信息
Department of Agricultural and Biological Engineering, Mississippi State University, 39762, Mississippi State, MS, USA.
出版信息
Cytotechnology. 2006 Mar;50(1-3):141-62. doi: 10.1007/s10616-005-5507-z. Epub 2006 Jun 30.
Abstract
Gene therapy is a promising technology for the treatment of several acquired and inherited diseases. However, for gene therapy to be a commercial and clinical success, scalable cell culture processes must be in place to produce the required amount of viral vectors to meet market demand. Each type of vector has its own distinct characteristics and consequently its own challenges for production. This article reviews the current technology that has been developed for the efficient, large-scale manufacture of retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus vectors.
摘要
基因治疗是治疗多种获得性和遗传性疾病的一种有前途的技术。然而，为了使基因治疗在商业和临床方面取得成功，必须建立可扩展的细胞培养工艺，以生产出满足市场需求的所需数量的病毒载体。每种类型的载体都有其独特的特点，因此在生产方面也有其自身的挑战。本文综述了目前已开发的用于高效、大规模生产逆转录病毒、慢病毒、腺病毒、腺相关病毒和单纯疱疹病毒载体的技术。
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