Graduate School of Systems Life Sciences, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Cytotechnology. 2008 Jun;57(2):161-8. doi: 10.1007/s10616-008-9128-1. Epub 2008 Feb 12.
Epigenetic modifications of histone play important roles for regulation of cell activity, such as cell division, cell death, and cell differentiation. A SET domain consisting of about 130 amino acids has lysine methyltransferase activity in the presence of the cosubstrate S-adenosyl-methionine. More than 60 SET domain-containing proteins have been predicted in various organisms. One of them, the SMYD family genes which contain a SET domain and a zinc-finger MYND domain are reported to regulate cell cycle and muscle formation. Here we examined the expression and function of smyd1 and 2 in Xenopus. smyd1 and 2 were expressed in various muscle tissues. While smyd1 expression was observed mainly in cardiac muscle and skeletal muscle, smyd2 expression was done abundantly in skeletal muscle and face region. Moreover, by loss-of-function experiments using antisense morpholino oligonucleotides, it was suggested that smyd1 and 2 related to muscle cells differentiation.
组蛋白的表观遗传修饰对于细胞活动的调节起着重要作用，如细胞分裂、细胞死亡和细胞分化。SET 结构域由大约 130 个氨基酸组成，在共底物 S-腺苷甲硫氨酸的存在下具有赖氨酸甲基转移酶活性。在各种生物体中已经预测到超过 60 个含有 SET 结构域的 SET 结构域蛋白。其中之一，SMYD 家族基因含有 SET 结构域和锌指 MYND 结构域，据报道可调节细胞周期和肌肉形成。在这里，我们研究了 smyd1 和 2 在非洲爪蟾中的表达和功能。smyd1 和 2 在各种肌肉组织中表达。虽然 smyd1 的表达主要在心肌和骨骼肌中观察到，但 smyd2 的表达在骨骼肌和面部区域中大量进行。此外，通过使用反义 morpholino 寡核苷酸的功能丧失实验，表明 smyd1 和 2 与肌肉细胞分化有关。
