Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, Brasil,
Cytotechnology. 2008 May;57(1):83-91. doi: 10.1007/s10616-008-9141-4. Epub 2008 Mar 2.
Gene expression in animal cells allows large scale production of proteins used for either structure and function studies or therapeutic purposes. Maximizing recombinant protein production is necessary to optimize cell growth and protein expression. Some studies have demonstrated the presence of pharmacologically active substances in insect hemolymph. In this work, we have identified and purified a protein from Lonomia obliqua hemolymph able to increase the production of the rabies virus glycoprotein, expressed in Drosophila melanogaster S2 cells, by about 59%.
动物细胞中的基因表达允许大规模生产用于结构和功能研究或治疗目的的蛋白质。最大限度地提高重组蛋白的产量对于优化细胞生长和蛋白质表达是必要的。一些研究已经证明了昆虫血淋巴中存在有药理活性的物质。在这项工作中，我们从 Lonomia obliqua 血淋巴中鉴定和纯化了一种蛋白，该蛋白能够使在 Drosophila melanogaster S2 细胞中表达的狂犬病病毒糖蛋白的产量增加约 59%。
