Greco Katia N, Mendonça Rita Maria Z, Moraes Roberto H P, Mancini Dalva A P, Mendonça Ronaldo Z
Laboratório de Parasitologia e Entomologia, Instituto Butantan, São Paulo, Brazil.
Antiviral Res. 2009 Oct;84(1):84-90. doi: 10.1016/j.antiviral.2009.07.018. Epub 2009 Aug 7.
Potent antiviral activity against measles, influenza and polio viruses was observed in the hemolymph of Lonomia obliqua. The antiviral protein responsible for this activity was isolated, purified by gel filtration chromatography using a gel filtration column system (Superdex 75) and further fractionated using a Resource-Q ion exchange column system. Experiments with the purified protein led to a 157-fold reduction (from 3.3+/-1.25 x 10(7) to 2.1+/-1.5 x 10(5) TCID(50)mL(-1)) in measles virus production and a 61-fold reduction (from 2.8+/-1.08 x 10(9) to 4.58+/-1.42 x 10(7)mL(-1)) in polio virus production. Heating and freezing seem to have no influence over its antiviral activity. Also, the protein does not display virucidal activity and does not act on receptors on the cell membrane. The observations suggest an intracellular mechanism of action and that the protein may act as a constitutive agent that affects the innate antiviral immune response.
在斜带毒蛾的血淋巴中观察到了对麻疹病毒、流感病毒和脊髓灰质炎病毒的强大抗病毒活性。负责这种活性的抗病毒蛋白被分离出来，通过使用凝胶过滤柱系统（Superdex 75）的凝胶过滤色谱法进行纯化，并进一步使用Resource-Q离子交换柱系统进行分级分离。对纯化后的蛋白进行的实验使麻疹病毒产量降低了157倍（从3.3±1.25×10⁷降至2.1±1.5×10⁵TCID₅₀mL⁻¹），脊髓灰质炎病毒产量降低了61倍（从2.
