Bioprocessing Technology Institute, Agency for Science Technology and Research, A*STAR, 20 Biopolis Way #06-01, 138668, Centros Singapore,
Cytotechnology. 2004 Jul;45(3):107-15. doi: 10.1007/s10616-004-6173-2.
Insulin is involved in a number of cellular functions, including the stimulation of cell growth, cell cycle progression and glucose uptake and is a common protein supplement in serum-free mammalian cell culture media. However, several trace metals have previously been reported to exhibit insulin-like effects on specific cell types. As a step towards developing chemically-defined, protein-free media for mammalian cells, we tested the effectiveness of five trace metals (cadmium, nickel, lithium, vanadium and zinc) as a replacement for insulin. Four cell lines of biotechnological relevance were used, including the hybridoma CRL1606, the myeloma NS0, and the Chinese hamster ovary cell lines CHO-IFN and CHO-K1. Zinc was found to be an effective insulin replacement for the hybridoma, myeloma and CHO-K1 cells. Cell growth, cell cycle progression and antibody production was not affected by the substitution. Furthermore, no adaptation procedure was required.
胰岛素参与许多细胞功能，包括刺激细胞生长、细胞周期进程和葡萄糖摄取，并且是无血清哺乳动物细胞培养物中的常见蛋白质补充物。然而，以前有报道称，一些痕量金属对特定细胞类型表现出类似胰岛素的作用。为了朝着开发用于哺乳动物细胞的化学定义、无蛋白质的培养基迈出一步，我们测试了五种痕量金属（镉、镍、锂、钒和锌）作为胰岛素替代物的有效性。使用了四种具有生物技术相关性的细胞系，包括杂交瘤 CRL1606、骨髓瘤 NS0 以及中国仓鼠卵巢细胞系 CHO-IFN 和 CHO-K1。锌被发现是杂交瘤、骨髓瘤和 CHO-K1 细胞的有效胰岛素替代品。细胞生长、细胞周期进程和抗体产生不受替代的影响。此外，不需要适应程序。
