重组蛋白在无血清培养基开发中的作用。
The role of recombinant proteins in the development of serum-free media.
机构信息
National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland,
出版信息
Cytotechnology. 2006 Mar;50(1-3):49-56. doi: 10.1007/s10616-006-9002-y. Epub 2006 Aug 5.
Abstract
Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived. Given recent concerns about TSEs (transmissible spongiform encephalopathies) and other adventitious agents, the drive in media formulations must be towards elimination of animal-origin materials while maintaining cell line productivity. The progress made towards removing animal-derived components and the use of recombinant proteins in serum-free media for mammalian cells is reviewed.
摘要
无血清培养基的早期发展导致了各种配方的出现，其中通常在血清中提供并对生长必需的成分（胰岛素、转铁蛋白、脂质补充剂、微量元素）和定义不明确的成分（提取物、水解物）被添加到定义明确的基础培养基中。这些添加剂大多来自动物。鉴于最近对 TSE（传染性海绵状脑病）和其他外来因子的关注，在培养基配方中必须朝着消除动物源材料的方向发展，同时保持细胞系的生产力。本文综述了在无血清培养基中去除动物源性成分并使用重组蛋白用于哺乳动物细胞的进展。
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本文引用的文献
1
Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.无血清生长培养基，用于搅拌式生物反应器中培养多种哺乳动物细胞。
Cytotechnology. 1988 Nov;1(4):319-29. doi: 10.1007/BF00365077.
2
Evaluation of the serum-free medium MDSS2 for the production of poliovirus on vero cells in bioreactors.评价无血清培养基 MDSS2 在生物反应器中生产脊髓灰质炎病毒在vero 细胞上的应用。
Cytotechnology. 1997 Nov;25(1-3):35-44. doi: 10.1023/A:1007999313566.
3
The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses.新型无动物蛋白培养基 MDSS2N 支持细胞生长和各种病毒的生产。
Cytotechnology. 1999 Jul;30(1-3):191-201. doi: 10.1023/A:1008021317639.
4
Virus contaminations of cell cultures - A biotechnological view.病毒污染细胞培养物——生物技术视角。
Cytotechnology. 2002 Jul;39(2):91-116. doi: 10.1023/A:1022969101804.
5
Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures.评价胰岛素模拟痕量金属作为哺乳动物细胞培养中的胰岛素替代品。
Cytotechnology. 2004 Jul;45(3):107-15. doi: 10.1007/s10616-004-6173-2.
6
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Cytotechnology. 2005 Jan;47(1-3):37-49. doi: 10.1007/s10616-005-3775-2.
7
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Biotechnol Bioeng. 1997 Nov 20;56(4):353-60. doi: 10.1002/(SICI)1097-0290(19971120)56:4<353::AID-BIT1>3.0.CO;2-N.
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