National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland,
Cytotechnology. 2006 Mar;50(1-3):49-56. doi: 10.1007/s10616-006-9002-y. Epub 2006 Aug 5.
Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived. Given recent concerns about TSEs (transmissible spongiform encephalopathies) and other adventitious agents, the drive in media formulations must be towards elimination of animal-origin materials while maintaining cell line productivity. The progress made towards removing animal-derived components and the use of recombinant proteins in serum-free media for mammalian cells is reviewed.
无血清培养基的早期发展导致了各种配方的出现，其中通常在血清中提供并对生长必需的成分（胰岛素、转铁蛋白、脂质补充剂、微量元素）和定义不明确的成分（提取物、水解物）被添加到定义明确的基础培养基中。这些添加剂大多来自动物。鉴于最近对 TSE（传染性海绵状脑病）和其他外来因子的关注，在培养基配方中必须朝着消除动物源材料的方向发展，同时保持细胞系的生产力。本文综述了在无血清培养基中去除动物源性成分并使用重组蛋白用于哺乳动物细胞的进展。
