Cell Culture Research and Development, GIBCO/Invitrogen Corporation, Grand Island, NY, 14072, U.S.A..
Cytotechnology. 2001 Jul;36(1-3):33-9. doi: 10.1023/A:1014045104525.
To overcome limitations of conventional milling technology, we investigated the application of fluid bed granulation for the production of dry-form nutrient media. Serum-free, protein-free and chemically-defined specialty media were produced in granulated format and compared with identical formulations manufactured by conventional methods. HPLC analysis of multiple lots of granulated materials demonstrated that biochemical constituents were precisely and homogeneously distributed throughout the granules and that nutrient levels were comparable to conventional formats. Comparison of medium performance in cell proliferation and biological production assays demonstrated equivalence with reference media. The fluid bed granulation process meets pharmaceutical quality requirements and may be applied to a broad range of nutrient formulations required for bioproduction applications.
为了克服传统制粒技术的局限性，我们研究了流化床制粒在生产干式营养培养基中的应用。以颗粒形式生产无血清、无蛋白和化学定义的特种培养基，并与通过传统方法生产的相同配方进行比较。对多批颗粒材料的 HPLC 分析表明，生化成分在颗粒中精确且均匀地分布，并且营养水平与传统配方相当。细胞增殖和生物生产测定中培养基性能的比较表明与参比培养基等效。流化床制粒工艺符合药物质量要求，可应用于生物生产应用所需的广泛营养配方。
