BioTherapeutics Research Group, Robarts Research Institute, 100 Perth Drive, P.O. Box 5015, N6A 5K8, London, ON, Canada.
Cytotechnology. 2005 Jun;48(1-3):79-88. doi: 10.1007/s10616-005-3795-y.
In light of the recent detection of BSE in North America and its endemic nature in other regions of the world, there is a real need to employ cell culture conditions that do not require any animal-derived material. Here we report the use of an ultra-low protein serum-free medium (VP-SFM, Invitrogen) for the amplification of poxviruses in primary chicken embryo fibroblasts (CEF). We compared the amplification of four different poxviruses (canarypox, modified Ankara Virus (MVA), vaccinia virus strain Copenhagen and myxoma strain Lausanne) in three different media: DMEM 10%, DMEM 2% and serum-free medium VP-SFM. VP-SFM is a serum-free, ultra-low protein medium containing no proteins or peptides of human or animal origin designed to support the replication of viruses and the production of recombinant proteins and monoclonal antibodies. Our results show that high titre poxvirus stocks can be prepared in VP-SFM equivalent to that prepared in serum containing medium.
鉴于近期在北美发现疯牛病以及其在世界其他地区的流行，我们确实需要使用不使用任何动物源性材料的细胞培养条件。在这里，我们报告了使用超低蛋白无血清培养基 (VP-SFM，Invitrogen) 在原代鸡胚成纤维细胞 (CEF) 中扩增痘病毒。我们比较了在三种不同培养基中扩增四种不同痘病毒（金丝雀痘病毒、改良安卡拉病毒 (MVA)、牛痘病毒株哥本哈根和兔粘液瘤病毒株洛桑）的效果：DMEM 10%、DMEM 2% 和无血清培养基 VP-SFM。VP-SFM 是一种无血清、超低蛋白培养基，不含任何人类或动物来源的蛋白质或肽，旨在支持病毒的复制以及重组蛋白和单克隆抗体的生产。我们的结果表明，VP-SFM 中可以制备高滴度的痘病毒，其效果与含血清培养基中制备的相当。
