在流化床生物反应器中高密度和高生产力培养中国仓鼠卵巢细胞。
High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor.
机构信息
Human Genome Sciences, Inc., 9410 Key West Ave., Rockville, MD, 20850, USA.
出版信息
Cytotechnology. 1999 May;29(3):215-20. doi: 10.1023/A:1008064217040.
A recombinant Chinese hamster ovary clone was cultivated in a 2L Cytopilot Mini fluidized bed bioreactor using Cytoline 1 microcarriers and a 10L B. Braun stirred tank bioreactor with Cytodex 1 microcarriers. Cytoline 1 is a macroporous polyethylene microcarrier and Cytodex 1 is a solid DEAE-dextran microcarrier. Cytoline 1 microcarriers in the fluidized bed bioreactor were gently mixed by an uplifting flow. Circulation and sparging in Cytopilot Mini were separated from the fluidized microcarrier bed. Cytopilot Mini bioreactor with Cytoline 1 microcarriers offered 2.3 times more surface area than the stirred tank bioreactor. The 2L fluidized bed bioreactor accommodated approximately half the cells in the 10L stirred tank bioreactor. Moreover, Cytopilot Mini had approximately three times more product output rate and 5.5 times higher specific productivity than the stirred tank bioreactor.
一个重组中国仓鼠卵巢克隆细胞在 2L Cytopilot 小型流化床生物反应器中使用 Cytoline 1 微载体和 10L B. Braun 搅拌槽生物反应器中的 Cytodex 1 微载体进行培养。Cytoline 1 是一种大孔聚乙烯微载体，Cytodex 1 是一种固体 DEAE-葡聚糖微载体。流化床生物反应器中的 Cytoline 1 微载体通过提升流轻柔混合。Cytopilot Mini 中的循环和通气与流化微载体床分离。装有 Cytoline 1 微载体的 Cytopilot Mini 生物反应器的表面积比搅拌槽生物反应器大 2.3 倍。2L 流化床生物反应器容纳的细胞数量约为 10L 搅拌槽生物反应器的一半。此外，Cytopilot Mini 的产物产率比搅拌槽生物反应器高约 3 倍，比搅拌槽生物反应器高 5.5 倍。
Zotero 插件