Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Cytotechnology. 1999 Jul;30(1-3):49-57. doi: 10.1023/A:1008093404237.
Understanding the relationships between cell cycle and protein expression is critical to the optimisation of media and environmental conditions for successful commercial operation of animal cell culture processes. Using flow cytometry for the analysis of the early phases of synchronised batch cultures, the dependency of product expression on cell cycle related events has been evaluated in a recombinant CHO cell line. Although the production of recombinant protein is initially found to be cell cycle related, the maximum specific protein productivity is only achieved at a later stage of the exponential phase which also sees a maximum in the intracellular protein concentration. Subsequent work suggests that it is the batch phase/medium composition of cultures which is the major determinant of maximum specific productivity in this cell line. Furthermore the effect of the positive association between S phase and specific productivity is subordinate to the effect of batch phase/medium composition on the specific productivity of batch cultures.
理解细胞周期与蛋白质表达之间的关系，对于优化动物细胞培养过程的介质和环境条件以实现成功的商业运营至关重要。使用流式细胞术分析同步批培养的早期阶段，评估了细胞周期相关事件对重组 CHO 细胞系中产物表达的影响。尽管最初发现重组蛋白的产生与细胞周期有关，但最大比蛋白产率仅在指数期的后期达到，此时细胞内蛋白浓度也达到最大值。随后的研究表明，培养物的批次阶段/培养基组成是该细胞系中最大比蛋白产率的主要决定因素。此外，S 期与比蛋白产率之间的正相关性的影响次于批次阶段/培养基组成对批培养物的比蛋白产率的影响。
