al-Rubeai M
Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, UK.
Adv Biochem Eng Biotechnol. 1998;59:225-49. doi: 10.1007/BFb0102300.
The importance of apoptosis in cell culture has now been widely recognised. Recent work has shown that this process is widely manifested during the in vitro cultivation of commercially important mammalian cell lines. In this review I summarise what is now known of the characteristics, significance and regulatory mechanisms of apoptosis. As the process of cell proliferation and cell death are now considered intimately related, particular attention is paid to highlight the progress and opportunities in the field of cell culture engineering. The strategies that have been undertaken to prevent the induction of apoptosis in cell culture and those which have been suggested as possibilities to improve culture productivity through the apoptosis route are discussed with given examples.
细胞凋亡在细胞培养中的重要性现已得到广泛认可。最近的研究表明，这一过程在商业上重要的哺乳动物细胞系的体外培养过程中广泛存在。在这篇综述中，我总结了目前已知的细胞凋亡的特征、意义和调控机制。由于现在认为细胞增殖和细胞死亡过程密切相关，因此特别关注突出细胞培养工程领域的进展和机遇。文中通过实例讨论了为防止细胞培养中诱导凋亡而采取的策略，以及那些被认为有可能通过凋亡途径提高培养生产力的策略。
