Department of Cell Engineering, Institute of Biotechnology, 20 Dongdajie, Fengtai, Beijing, 100071, P.R. China.
Cytotechnology. 1999 Jul;30(1-3):143-7. doi: 10.1023/A:1008038609967.
Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant CHO cell line CL-11G producing prourokinase and hybridomas producing anti-prourokinase monoclonal antibody in Celligen 1.5 or 5 L bioreactor. The cell density obtained ranged from 1 to 2 x 107 cells mL-1. The yields of prourokinase and monoclonal antibody increased with increasing cell density. As the cells could spontaneously release from and reattach to porous microcarriers, it was very easy to scale-up the cultivation. Thus the bead to bead cell transfer method has been used to scale up the cultivation of CL-11G cells to a 20 L reactor-scale for the pilot production of prourokinase, and also to scale-up the culture of hybridomas for the production of monoclonal antibody for the purification of prourokinase.
使用多孔微载体 Cytopore 和低血清培养基补充剂 BIGBEF-3，我们成功地在 Celligen 1.5 或 5 L 生物反应器中培养了生产尿激酶原的重组 CHO 细胞系 CL-11G 和生产抗尿激酶原单克隆抗体的杂交瘤。获得的细胞密度范围为 1 到 2 x 107 个细胞/mL-1。尿激酶原和单克隆抗体的产量随细胞密度的增加而增加。由于细胞可以自发地从多孔微载体上释放并重新附着，因此很容易进行放大培养。因此，采用珠对珠细胞转移方法将 CL-11G 细胞的培养放大到 20 L 反应规模，用于尿激酶原的中试生产，也用于杂交瘤的培养放大，以生产用于纯化尿激酶原的单克隆抗体。
