一种新型多孔载体及其在悬浮细胞培养中的应用。

A new type porous carrier and its application to culture of suspension cells.

作者信息

Gotoh T, Honda H, Shiragami N, Unno H

机构信息

Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Cytotechnology. 1993;11(1):35-40. doi: 10.1007/BF00749055.

DOI:10.1007/BF00749055

PMID:7764053

Abstract

A new type porous carrier was fabricated from a mixture of sodium alginate, bovine serum albumin and sodium bicarbonate. The porous space of the carrier is an assembly of void spaces. The carrier was successfully applied to the cultivation of suspension animal cells. In the culture, while both cells and carriers were held in suspension, the cells were entrapped hydrodynamically into the void spaces in the carriers. A culture of hybridoma cells using this carrier resulted in a cell density up to 5.7 x 10(7) cells per ml-carrier.

摘要

一种新型多孔载体由海藻酸钠、牛血清白蛋白和碳酸氢钠的混合物制成。该载体的多孔空间是空隙空间的集合。该载体已成功应用于悬浮动物细胞的培养。在培养过程中，细胞和载体都保持悬浮状态，细胞通过流体动力学作用被困在载体的空隙空间中。使用这种载体培养杂交瘤细胞，细胞密度可达每毫升载体5.7×10⁷个细胞。