Department of Chemical Engineering, The University of Sydney, NSW, 2006, Australia.
Cytotechnology. 2000 Oct;34(1-2):71-82. doi: 10.1023/A:1008178029138.
The infection of insect cells with baculovirus was described in a mathematical model as a part of the structured dynamic model describing whole animal cell metabolism. The model presented here is capable of simulating cell population dynamics, the concentrations of extracellular and intracellularviral components, and the heterologous product titers. The model describes the whole processes of viral infection and theeffect of the infection on the host cell metabolism. Dynamic simulation of the model in batch and fed-batch mode gave goodagreement between model predictions and experimental data. Optimum conditions for insect cell culture and viral infectionin batch and fed-batch culture were studied using the model.
杆状病毒感染昆虫细胞被描述为描述整个动物细胞代谢的结构动态模型的一部分。本文提出的模型能够模拟细胞群体动力学、细胞外和细胞内病毒成分的浓度以及异源产物滴度。该模型描述了病毒感染的全过程及其对宿主细胞代谢的影响。在批式和补料分批培养模式下对模型进行动态模拟，模型预测与实验数据吻合良好。使用该模型研究了昆虫细胞培养和病毒感染在批式和补料分批培养中的最佳条件。
