Simulation of animal cell metabolism.

A simulation of hybridoma growth and antibody production has been developed. It is capable of simulating all major variables of interest (e.g., specific growth rate, cell yield, sugars and amino acids profile, and antibody yield). This simulation is the most complete reported to date including such factors as cell composition, media composition, substrate and product effects, osmolarity etc. The stimulation of a large range of experimental data for hybridomas illustrates that this simulation is a powerful tool in the rational assessment of factors influencing the growth and metabolism of hybridoma cells.