Barford J P, Phillips P J, Harbour C
Department of Chemical Engineering, University of Sydney, NSW, Australia.
Cytotechnology. 1992;10(1):63-74. doi: 10.1007/BF00376101.
A simulation of hybridoma growth and antibody production has been developed. It is capable of simulating all major variables of interest (e.g., specific growth rate, cell yield, sugars and amino acids profile, and antibody yield). This simulation is the most complete reported to date including such factors as cell composition, media composition, substrate and product effects, osmolarity etc. The stimulation of a large range of experimental data for hybridomas illustrates that this simulation is a powerful tool in the rational assessment of factors influencing the growth and metabolism of hybridoma cells.
已经开发了一种杂交瘤生长和抗体产生的模拟模型。它能够模拟所有主要的感兴趣变量（例如，比生长速率、细胞产量、糖和氨基酸谱以及抗体产量）。该模拟是迄今为止报道的最完整的，包括细胞组成、培养基组成、底物和产物效应、渗透压等因素。对大量杂交瘤实验数据的模拟表明，该模拟是合理评估影响杂交瘤细胞生长和代谢因素的有力工具。
