Swiss Federal Institute of Technology, ETH Zurich, Institute of Biotechnology, CH-8093, Zurich, Switzerland.
Cytotechnology. 1998 Nov;28(1-3):111-26. doi: 10.1023/A:1008037916674.
Contemporary basic research is rapidly revealing increasingly complex molecular regulatory networks which are often interconnected via key signal integrators. These connections among regulatory and catalytic networks often frustrate bioengineers as promising metabolic engineering strategies are bypassed by compensatory metabolic responses or cause unexpected, undesired outcomes such as apoptosis, product protein degradation or inappropriate post- translational modification. Therefore, for metabolic engineering to achieve greater success in mammalian cell culture processes and to become important for future applications such as gene therapy and tissue engineering, this technology must be enhanced to allow simultaneous, in cases conditional, reshaping of metabolic pathways to access difficult-to-attain cell states. Recent advances in this new territory of multigene metabolic engineering are intimately linked to the development of multicistronic expression technology which allows the simultaneous, and in some cases, regulated expression of several genes in mammalian cells. Here we review recent achievements in multicistronic expression technology in view of multigene metabolic engineering.
当代基础研究正在迅速揭示出越来越复杂的分子调控网络，这些网络通常通过关键信号整合器相互连接。这些调控和催化网络之间的联系常常令生物工程师感到沮丧，因为有前途的代谢工程策略被补偿性代谢反应所绕过，或者导致意想不到的、不期望的结果，如细胞凋亡、产物蛋白降解或不适当的翻译后修饰。因此，为了使代谢工程在哺乳动物细胞培养过程中取得更大的成功，并成为基因治疗和组织工程等未来应用的重要手段，这项技术必须得到加强，以允许在某些情况下对代谢途径进行同时的、有条件的重塑，以进入难以达到的细胞状态。在这个多基因代谢工程的新领域中，多顺反子表达技术的发展与多基因代谢工程密切相关，该技术允许在哺乳动物细胞中同时（在某些情况下是有调节的）表达几个基因。在这里，我们回顾了多顺反子表达技术在多基因代谢工程方面的最新进展。
