Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Curr Opin Biotechnol. 2017 Oct;47:67-82. doi: 10.1016/j.copbio.2017.06.007. Epub 2017 Jul 1.
Metabolic engineering has been playing increasingly important roles in developing microbial cell factories for the production of various chemicals and materials to achieve sustainable chemical industry. Nowadays, many tools and strategies are available for performing systems metabolic engineering that allows systems-level metabolic engineering in more sophisticated and diverse ways by adopting rapidly advancing methodologies and tools of systems biology, synthetic biology and evolutionary engineering. As an outcome, development of more efficient microbial cell factories has become possible. Here, we review recent advances in systems metabolic engineering tools and strategies together with accompanying application examples. In addition, we describe how these tools and strategies work together in simultaneous and synergistic ways to develop novel microbial cell factories.
代谢工程在开发微生物细胞工厂以生产各种化学品和材料方面发挥着越来越重要的作用，以实现可持续的化学工业。如今，有许多工具和策略可用于进行系统代谢工程，通过采用系统生物学、合成生物学和进化工程的快速发展方法和工具，可以以更复杂和多样化的方式进行系统水平的代谢工程。因此，开发更高效的微生物细胞工厂成为可能。在这里，我们综述了系统代谢工程工具和策略的最新进展及其伴随的应用实例。此外，我们还描述了这些工具和策略如何协同工作，以开发新型的微生物细胞工厂。
