Jenkins N, Parekh R B, James D C
Department of Biological Sciences, De Montfort University, Leicester, UK.
Nat Biotechnol. 1996 Aug;14(8):975-81. doi: 10.1038/nbt0896-975.
Glycosylation is the most extensive of all the posttranslational modifications, and has important functions in the secretion, antigenicity and clearance of glycoproteins. In recent years major advances have been made in the cloning of glycosyltransferase enzymes, in understanding the varied biological functions of carbohydrates, and in the accurate analysis of glycoprotein heterogeneity. In this review we discuss the impact of these advances on the choice of a recombinant host cell line, in optimizing cell culture processes, and in choosing the appropriate level of glycosylation analysis for each stage of product development.
糖基化是所有翻译后修饰中最为广泛的一种，并且在糖蛋白的分泌、抗原性及清除过程中发挥着重要作用。近年来，在糖基转移酶的克隆、对碳水化合物多种生物学功能的理解以及对糖蛋白异质性的精确分析方面均取得了重大进展。在本综述中，我们将探讨这些进展对重组宿主细胞系的选择、细胞培养过程优化以及为产品开发的每个阶段选择合适糖基化分析水平的影响。
