Panova Natalya G, Alexeev Cyril S, Polyakov Konstantin M, Gavryushov Sergei A, Kritzyn Anatoliy M, Mikhailov Sergey N
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Nucleosides Nucleotides Nucleic Acids. 2008 Dec;27(12):1211-4. doi: 10.1080/15257770802257895.
Substrate specificity of E. coli thymidine phosphorylase to pyrimidine nucleoside modified at 5'-, 3'-, and 2'-positions of sugar moiety has been studied. Equilibrium (K(eq)) and kinetics constants of phosphorolysis reaction of nucleosides were measured. The most important hydrogen bonds in enzyme-substrate complex have been determined.
