Swamy Malisetty V, Citineni Bhargava, Patlolla Jagan M R, Mohammed Altaf, Zhang Yuting, Rao Chinthalapally V
Department of Medicine, Hem-Onc Section, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Nutr Cancer. 2008;60 Suppl 1:81-9. doi: 10.1080/01635580802416703.
Pancreatic cancer BxPC-3 cells were exposed to curcumin, docosahexaenoic acid (DHA), or combinations of both and analyzed for proliferation and apoptosis. Pancreatic tumor xenografts were established by injecting BxPC-3 cells into each flank of nude mice. After the tumors reached a size of approximately 190-200 mm(3), animals were fed diets with or without 2,000 ppm curcumin in 18% corn oil or 15% fish oil + 3% corn oil for 6 more wk before assessing the tumor volume and expression of inducible nitric oxide synthase (iNOS), cyclooxygeanse-2 (COX-2), 5-lipoxinase (5-LOX), and p21. A synergistic effect was observed on induction of apoptosis (approximately sixfold) and inhibition of cell proliferation (approximately 70%) when cells were treated with curcumin (5 microM) together with the DHA (25 microM). Mice fed fish oil and curcumin showed a significantly reduced tumor volume, 25% (P < 0.04) and 43% (P < 0.005), respectively, and importantly, a combination of curcumin and fish oil diet showed > 72% (P < 0.0001) tumor volume reduction. Expression and activity of iNOS, COX-2, and 5-LOX are downregulated, and p21 is upregulated in tumor xenograft fed curcumin combined with fish oil diet when compared to individual diets. The preceding results evidence for the first time that curcumin combined with omega-3 fatty acids provide synergistic pancreatic tumor inhibitory properties.
将胰腺癌BxPC - 3细胞暴露于姜黄素、二十二碳六烯酸（DHA）或两者的组合中，然后分析其增殖和凋亡情况。通过将BxPC - 3细胞注射到裸鼠的双侧胁腹来建立胰腺肿瘤异种移植模型。当肿瘤大小达到约190 - 200立方毫米后，给动物喂食含或不含2000 ppm姜黄素的18%玉米油或15%鱼油 + 3%玉米油的饲料，持续6周，之后评估肿瘤体积以及诱导型一氧化氮合酶（iNOS）、环氧化酶 - 2（COX - 2）、5 - 脂氧合酶（5 - LOX）和p21的表达。当细胞用姜黄素（5微摩尔）与DHA（25微摩尔）共同处理时，观察到对细胞凋亡的诱导（约六倍）和细胞增殖的抑制（约70%）具有协同作用。喂食鱼油和姜黄素的小鼠肿瘤体积分别显著减少25%（P < 0.（此处原文有误，推测应为P < 0.04））和43%（P < 0.005），重要的是，姜黄素与鱼油饮食组合使肿瘤体积减少超过72%（P < 0.0001）。与单独饮食相比，喂食姜黄素与鱼油组合饮食的肿瘤异种移植中，iNOS、COX - 2和5 - LOX的表达及活性下调，p21上调。上述结果首次证明姜黄素与ω - 3脂肪酸联合具有协同抑制胰腺肿瘤的特性。
