Ermakova I T, Vinokurova N G, Zelenkova N F, Baskunov B P, Leont'evskiĭ A A
Mikrobiologiia. 2008 Sep-Oct;77(5):617-22.
A screening of lignin-degrading basidial fungi that can grow in the presence of thiomorpholine derivatives (the mixture of 1,4-perhydrothiazines) has been performed. Strain Bjerkandera adusta VKM F-3477 was shown to have the maximal rate of growth in the presence of these compounds, and its capacity for thiomorpholine degradation was studied. The methods of quantitative analysis of thiomorpholine and its degradation products on the basis of thin layer chromatography and high-performance liquid chromatography were developed. It was shown that the B. adusta strain did not utilize thiomorpholine as a carbon source but transformed it into thiomorpholine sulfoxide that accumulated in the medium. Mn peroxidase produced by B. adusta in the course of thiomorpholine transformation is not directly involved in its oxidation.
已对能在硫代吗啉衍生物（1,4-全氢噻嗪混合物）存在下生长的木质素降解担子菌进行了筛选。结果表明，菌株烟管菌VKM F-3477在这些化合物存在下具有最大生长速率，并对其硫代吗啉降解能力进行了研究。建立了基于薄层色谱和高效液相色谱的硫代吗啉及其降解产物的定量分析方法。结果表明，烟管菌菌株不利用硫代吗啉作为碳源，而是将其转化为在培养基中积累的硫代吗啉亚砜。烟管菌在硫代吗啉转化过程中产生的锰过氧化物酶并不直接参与其氧化过程。
