Robinson Tim, Nigam Poonam Singh
School of Biomedical Sciences, Faculty of Life and Health Sciences, University of Ulster, Coleraine, Northern Ireland, UK.
Appl Biochem Biotechnol. 2008 Dec;151(2-3):618-28. doi: 10.1007/s12010-008-8272-6. Epub 2008 May 22.
A strict screening strategy for microorganism selection was followed employing a number of white-rot fungi for the bioremediation of textile effluent, which was generated from one Ireland-based American textile industry. Finally, one fungus Bjerkandera adusta has been investigated in depth for its ability to simultaneously degrade and enrich the nutritional quality of highly coloured textile effluent-adsorbed barley husks through solid-state fermentation (SSF). Certain important parameters such as media requirements, moisture content, protein/biomass production and enzyme activities were examined in detail. A previously optimised method of dye desorption was employed to measure the extent of dye remediation through effluent decolorisation achieved as a result of fungal activity in SSF. B. adusta was capable of decolourising a considerable concentration of the synthetic dye effluent (up to 53%) with a moisture content of 80-85%. Protein enrichment of the fermented mass was achieved to the extent of 229 g/kg dry weight initial substrate used. Lignin peroxidase and laccase were found to be the two main enzymes produced during SSF of the dye-adsorbed lignocellulosic waste residue.
采用了严格的微生物筛选策略，选用了多种白腐真菌对一家位于爱尔兰的美国纺织工业产生的纺织废水进行生物修复。最后，深入研究了一种真菌——烟管菌，它能够通过固态发酵（SSF）同时降解并提高吸附了高色度纺织废水的大麦壳的营养质量。详细研究了一些重要参数，如培养基需求、水分含量、蛋白质/生物量产量和酶活性。采用先前优化的染料解吸方法来测量由于固态发酵中真菌活性导致的废水脱色而实现的染料修复程度。烟管菌在水分含量为80 - 85%时，能够使相当浓度的合成染料废水脱色（高达53%）。发酵产物的蛋白质富集量达到了所用初始底物干重的229 g/kg。在吸附了染料的木质纤维素废渣的固态发酵过程中，发现木质素过氧化物酶和漆酶是产生的两种主要酶。
