Thaa Bastian, Herrmann Andreas, Veit Michael
Department of Immunology and Molecular Biology Veterinary Faculty, Free University Berlin, Philippstr. 13, 10115 Berlin, Germany.
Virology. 2009 Jan 5;383(1):150-5. doi: 10.1016/j.virol.2008.10.001. Epub 2008 Nov 12.
The matrix protein M1, the organizer of assembly of influenza virus, interacts with other virus components and with cellular membranes. It has been proposed that M1 binding to lipids is mediated by its polybasic region, but this could hitherto not been investigated in vivo since M1 accumulates in the nucleus of transfected cells. We have equipped M1 with nuclear export signals and showed that the constructs are bound to cellular membranes. Exchange of the complete polybasic region and of further hydrophobic amino acids in its vicinity did not prevent association of M1 with membranes. We therefore suppose that M1 probably interacts with membranes via multiple binding sites.
基质蛋白M1是流感病毒组装的组织者，它与病毒的其他组分以及细胞膜相互作用。有人提出M1与脂质的结合是由其多碱性区域介导的，但由于M1在转染细胞的细胞核中积累，迄今为止无法在体内进行研究。我们给M1配备了核输出信号，并表明构建体与细胞膜结合。完整多碱性区域及其附近其他疏水氨基酸的交换并不妨碍M1与膜的结合。因此我们推测M1可能通过多个结合位点与膜相互作用。
