Schwacha Martin G, Daniel Tanjanika
Department of Surgery, Division of Trauma and Emergency Surgery, University of Texas Health Science Center at San Antonio, Mail Code 7740, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
Cytokine. 2008 Dec;44(3):328-34. doi: 10.1016/j.cyto.2008.09.001. Epub 2008 Nov 11.
Burn injury is associated with profound inflammation and activation of the innate immune system involving gammadelta T-cells. Similarly, Toll-like receptors (TLR) are associated with activation of the innate immune response; however, it is unclear whether TLR expression is altered in gammadelta T-cells after major burn injury. To study this, male C57BL/6 mice were subjected to burn injury (25% TBSA) and 1 or 7 days thereafter, blood and spleen cells were isolated and subjected to FACs analysis for TLRs and other phenotypic markers (gammadelta TCR, alphabeta TCR, CD69, CD120b). A marked increase in the number of circulating gammadelta T-cells was observed at 24h post-burn (14% vs. 4%) and a higher percentage of these cells expressed TLR-2. TLR-4 expression was also increased post-burn, but to a lesser degree. These changes in TLR expression were not associated with altered CD69 or CD120b expression in gammadelta T-cells. The mobilization of, and increased TLR expression in, gammadelta T-cells was transient, as phenotypic changes were not evident at 7 days post-burn or in gammadelta T-cells from the circulation or spleen. The increases in TLR expression were not observed in alphabeta T-cells after burn injury. In conclusion, 24h after burn injury mobilization of gammadelta T-cells with increased TLR expression was observed. This finding suggests that this unique T-cell population is critical in the innate immune response to injury, possibly through the recognition of danger signals by TLRs.
烧伤与严重炎症以及涉及γδ T细胞的固有免疫系统激活相关。同样，Toll样受体（TLR）与固有免疫反应的激活有关；然而，严重烧伤后γδ T细胞中TLR表达是否改变尚不清楚。为了研究这一点，对雄性C57BL/6小鼠进行烧伤（25%体表面积），并在烧伤后1天或7天，分离血液和脾脏细胞，对TLR和其他表型标志物（γδ TCR、αβ TCR、CD69、CD120b）进行流式细胞术分析。烧伤后24小时观察到循环γδ T细胞数量显著增加（14%对4%），并且这些细胞中较高比例表达TLR-2。烧伤后TLR-4表达也增加，但程度较小。γδ T细胞中TLR表达的这些变化与γδ T细胞中CD69或CD120b表达的改变无关。γδ T细胞的动员和TLR表达增加是短暂的，因为在烧伤后7天或循环或脾脏中的γδ T细胞中表型变化不明显。烧伤后αβ T细胞中未观察到TLR表达增加。总之，烧伤后24小时观察到γδ T细胞动员且TLR表达增加。这一发现表明，这一独特的T细胞群体在对损伤的固有免疫反应中至关重要，可能是通过TLR识别危险信号来实现的。
