Peterson R N, Freund M
Fertil Steril. 1977 Mar;28(3):257-61.
Cytochalasin B inhibits the motility and metabolism of washed human spermatozoa at low concentrations (20 to 200 mum). Spermatozoal motility (primarily the frequency of flagellar contraction) declines slowly after addition of the antibiotic but is not abolished even after treatment for several hours. The addition of caffeine or dibutyrylcyclic adenosine 3' :5'-monophosphate to washed sperm suspensions increases the percentage of motile cells, the frequency of flagellar contraction, and the rate of glycolysis. These effects are blocked by cytochalasin B. However, cytochalasin B-treated spermatozoa regain their responsiveness to these agents when the antibiotic is washed out of supporting media. These effects are discussed in terms of an interaction of cytochalasin B with the sperm plasma membrane.
细胞松弛素B在低浓度（20至200μm）时可抑制洗涤后的人类精子的活力和代谢。添加抗生素后，精子活力（主要是鞭毛收缩频率）缓慢下降，但即使处理数小时也不会完全消失。向洗涤后的精子悬液中添加咖啡因或二丁酰环腺苷3':5'-单磷酸可增加活动细胞的百分比、鞭毛收缩频率和糖酵解速率。这些作用可被细胞松弛素B阻断。然而，当将抗生素从支持培养基中洗去时，经细胞松弛素B处理的精子会恢复对这些试剂的反应性。根据细胞松弛素B与精子质膜的相互作用对这些作用进行了讨论。
