在存在和不存在3'非翻译区的情况下，登革病毒5'端溶液结构中的构象变化。
Conformational changes in the solution structure of the dengue virus 5' end in the presence and absence of the 3' untranslated region.
作者信息
Polacek Charlotta, Foley Jonathan E, Harris Eva
机构信息
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, Berkeley, California 94720-7354, USA.
出版信息
J Virol. 2009 Jan;83(2):1161-6. doi: 10.1128/JVI.01362-08. Epub 2008 Nov 12.
Abstract
Dengue virus (DENV) is an approximately 10.7-kb positive-sense RNA virus that circularizes via RNA-RNA interactions between sequences in the 5' and 3' terminal regions. Complementarity between the cyclization sequence (CS) and the upstream AUG region (UAR) has been shown to be necessary for viral replication. Here, we present the solution structure of the 5' end of DENV type 2 in the presence and absence of the 3' end. We demonstrate that hybridization between the 5' and 3' CSs is independent of the UAR while the 5' UAR-3' UAR hybridization is dependent upon the 5' CS-3' CS interaction.
摘要
登革病毒（DENV）是一种约10.7千碱基的正链RNA病毒，它通过5'和3'末端区域序列之间的RNA - RNA相互作用形成环状。环化序列（CS）与上游AUG区域（UAR）之间的互补性已被证明对病毒复制是必需的。在这里，我们展示了在有和没有3'末端的情况下登革热2型病毒5'末端的溶液结构。我们证明5'和3' CS之间的杂交独立于UAR，而5' UAR - 3' UAR杂交则依赖于5' CS - 3' CS相互作用。
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