Saxena Manvendra, Coloe Peter J, Smooker Peter M
School of Applied Sciences, RMIT University, Bundoora, Victoria, Australia.
Clin Vaccine Immunol. 2009 Jan;16(1):78-87. doi: 10.1128/CVI.00253-08. Epub 2008 Nov 12.
Attenuated Salmonella strains are currently in production as vaccines for protection of animals against salmonellosis. Such commercial strains offer the potential to deliver heterologous antigen to protect animals against other diseases. One vaccine strain, attenuated Salmonella enterica serovar Typhimurium (STM-1), was tested for the ability to deliver ovalbumin and to induce immune responses in mice. Two vaccine trials were performed testing the influence of promoter choice, the location of the encoding DNA (plasmid or chromosome), and the effect of preexisting homologous or heterologous immunity. The results demonstrated that humoral and T-cell responses were induced from either of two promoters, from either the plasmid or the chromosome, and that preexposure to the empty homologous vector, STM-1, or the heterologous vector, S. enterica serovar Enteritidis, had no detrimental effect on subsequent antigen-specific responses. In the case of homologous preexposure, responses were generally greater, and this was correlated with an increased uptake of Salmonella by macrophages in vitro after opsonization with immune sera.
减毒沙门氏菌菌株目前正在生产中，用作保护动物免受沙门氏菌病感染的疫苗。此类商业菌株具有递送异源抗原以保护动物免受其他疾病感染的潜力。对一种疫苗菌株——减毒肠炎沙门氏菌鼠伤寒血清型（STM-1）——进行了测试，以评估其递送卵清蛋白并在小鼠体内诱导免疫反应的能力。进行了两项疫苗试验，测试启动子选择、编码DNA（质粒或染色体）位置的影响，以及预先存在的同源或异源免疫的影响。结果表明，两种启动子中的任何一种、质粒或染色体中的任何一种都能诱导体液和T细胞反应，并且预先暴露于空的同源载体STM-1或异源载体肠炎沙门氏菌肠炎血清型对随后的抗原特异性反应没有不利影响。在同源预先暴露的情况下，反应通常更强，这与用免疫血清调理后巨噬细胞在体外对沙门氏菌摄取的增加相关。
