ThinPrep制片法对通过聚合酶链反应检测直肠样本中人类乳头瘤病毒及其基因分型的影响。
Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR.
作者信息
Tabrizi Sepehr N, Stevens Matthew P, van Leeuwen Marina T, Vajdic Claire M, Botes Leon P, Medley Gabriele, Hillman Richard, Grulich Andrew, Garland Suzanne M
机构信息
Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia.
出版信息
J Clin Microbiol. 2009 Jan;47(1):227-9. doi: 10.1128/JCM.01518-08. Epub 2008 Nov 12.
Abstract
Specimen-to-specimen carryover during ThinPrep slide preparation was evaluated by comparing human papillomavirus genotypes detected prior and subsequent to the ThinPrep processing of 121 PreservCyt samples. Overall, 52 samples generated concordant genotypes and 38 had additional and 21 had fewer genotypes postprocessing. PreservCyt samples should be aliquoted for PCR testing prior to ThinPrep processing.
摘要
通过比较121份PreservCyt样本在ThinPrep处理前后检测到的人乳头瘤病毒基因型，评估了ThinPrep玻片制备过程中样本间的残留情况。总体而言，52个样本产生了一致的基因型，38个样本在处理后有额外的基因型，21个样本在处理后的基因型较少。PreservCyt样本应在ThinPrep处理前进行分装以用于PCR检测。
相似文献
1
Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR.
J Clin Microbiol. 2009 Jan;47(1):227-9. doi: 10.1128/JCM.01518-08. Epub 2008 Nov 12.
2
Generic microtiter plate assay for triaging clinical specimens prior to genotyping of human papillomavirus DNA via consensus PCR.
J Clin Microbiol. 2011 Nov;49(11):3977-9. doi: 10.1128/JCM.05646-11. Epub 2011 Sep 21.
3
Comparison of two PCR-based human papillomavirus genotyping methods.
J Clin Microbiol. 2008 Oct;46(10):3437-45. doi: 10.1128/JCM.00620-08. Epub 2008 Aug 20.
4
Detection and typing of low-risk human papillomavirus genotypes HPV 6, HPV 11, HPV 42, HPV 43 and HPV 44 by polymerase chain reaction and restriction fragment length polymorphism.
J Virol Methods. 2010 Oct;169(1):215-8. doi: 10.1016/j.jviromet.2010.07.007. Epub 2010 Jul 15.
5
Asymmetric GP5+/6+ PCR and hybridization with fluorescence polarization assay of 15 human papillomavirus genotypes in clinical samples.
J Clin Virol. 2009 Feb;44(2):106-10. doi: 10.1016/j.jcv.2008.12.003. Epub 2009 Jan 13.
6
Application of a multiplex PCR to cervical cells collected by a paper smear for the simultaneous detection of all mucosal human papillomaviruses (HPVs) and typing of high-risk HPV types 16 and 18.
J Med Microbiol. 2010 Nov;59(Pt 11):1303-1310. doi: 10.1099/jmm.0.019240-0. Epub 2010 Aug 5.
7
Improving Laboratory Efficiency by Automation of Preanalytic Processing of ThinPrep Specimens for Real-Time PCR High-Risk HPV Testing.
J Lab Autom. 2016 Jun;21(3):432-8. doi: 10.1177/2211068215569347. Epub 2015 Feb 11.
8
Effect of preanalytical processing of ThinPrep specimens on detection of high-risk human papillomavirus by the Aptima HPV assay.
J Clin Microbiol. 2014 May;52(5):1448-52. doi: 10.1128/JCM.03624-13. Epub 2014 Feb 19.
9
Development and Validation of a Preanalytic Procedure for Performing the cobas HPV Test in SurePath Preservative Fluid.
J Mol Diagn. 2017 Mar;19(2):288-294. doi: 10.1016/j.jmoldx.2016.10.003. Epub 2016 Dec 30.
10
Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR.
J Clin Virol. 2008 Aug;42(4):361-7. doi: 10.1016/j.jcv.2008.03.018. Epub 2008 May 2.
引用本文的文献
1
Transplant Recipients and Anal Neoplasia Study: Design, Methods, and Participant Characteristics of a Prevalence Study.
Transplant Direct. 2019 Mar 4;5(4):e434. doi: 10.1097/TXD.0000000000000873. eCollection 2019 Apr.
2
The Study of the Prevention of Anal Cancer (SPANC): design and methods of a three-year prospective cohort study.
BMC Public Health. 2013 Oct 9;13:946. doi: 10.1186/1471-2458-13-946.
本文引用的文献
1
Validation of an automated detection platform for use with the roche linear array human papillomavirus genotyping test.
J Clin Microbiol. 2008 Nov;46(11):3813-6. doi: 10.1128/JCM.01169-08. Epub 2008 Sep 10.
2
Type-specific reproducibility of the Roche linear array HPV genotyping test.
J Clin Virol. 2008 Aug;42(4):412-4. doi: 10.1016/j.jcv.2008.03.004. Epub 2008 Apr 18.
3
Significance and possible causes of false-negative results of reflex human Papillomavirus infection testing.
Cancer. 2007 Jun 25;111(3):154-9. doi: 10.1002/cncr.22688.
4
Evaluation of the SPF10-INNO LiPA human papillomavirus (HPV) genotyping test and the roche linear array HPV genotyping test.
J Clin Microbiol. 2006 Sep;44(9):3122-9. doi: 10.1128/JCM.00517-06.
5
Assessment of MagNA pure LC extraction system for detection of human papillomavirus (HPV) DNA in PreservCyt samples by the Roche AMPLICOR and LINEAR ARRAY HPV tests.
J Clin Microbiol. 2006 Jul;44(7):2428-33. doi: 10.1128/JCM.02608-05.
6
Human papillomavirus genotyping using a modified linear array detection protocol.
J Virol Methods. 2006 Jul;135(1):124-6. doi: 10.1016/j.jviromet.2006.02.007. Epub 2006 Mar 27.
7
Evaluation of a modified reverse line blot assay for detection and typing of human papillomavirus.
Am J Clin Pathol. 2005 Jun;123(6):896-9. doi: 10.1309/MPER-NJ0G-62RE-CHCQ.
8
Liquid-based cervical cytologic smear study and conventional Papanicolaou smears: a metaanalysis of prospective studies comparing cytologic diagnosis and sample adequacy.
Am J Obstet Gynecol. 2001 Aug;185(2):308-17. doi: 10.1067/mob.2001.116736.
9
Comparison of three management strategies for patients with atypical squamous cells of undetermined significance: baseline results from a randomized trial.
J Natl Cancer Inst. 2001 Feb 21;93(4):293-9. doi: 10.1093/jnci/93.4.293.
10
Potential for routine concurrent determination of chlamydia and cervical abnormalities by single fluid-based sampling.
Prim Care Update Ob Gyns. 1998 Jul 1;5(4):149-150. doi: 10.1016/s1068-607x(98)00030-4.
Zotero 插件