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用于罗氏线性阵列人乳头瘤病毒基因分型检测的自动检测平台的验证

Validation of an automated detection platform for use with the roche linear array human papillomavirus genotyping test.

作者信息

Stevens Matthew P, Garland Suzanne M, Tabrizi Sepehr N

机构信息

Department of Microbiology, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia.

出版信息

J Clin Microbiol. 2008 Nov;46(11):3813-6. doi: 10.1128/JCM.01169-08. Epub 2008 Sep 10.

Abstract

An automated platform (BeeBlot) was evaluated in parallel with the recommended protocol for the hybridization and detection steps of the Roche Linear Array human papillomavirus (HPV) genotyping test using DNA from 143 cervical specimens. Genotyping profiles showed 100% concordance between the methods, suggesting that automation could complement the Roche Linear Array for enhanced speed and reproducibility.

摘要

使用来自143份宫颈标本的DNA,将一个自动化平台(BeeBlot)与罗氏线性阵列人乳头瘤病毒(HPV)基因分型检测杂交和检测步骤的推荐方案进行了平行评估。基因分型结果显示两种方法之间的一致性为100%,这表明自动化可以补充罗氏线性阵列,以提高速度和可重复性。

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Evaluation of linear array human papillomavirus genotyping using automatic optical imaging software.
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