Ozturk K, Ozyurt H, Somay A, Karaca C
Department of Orthopedic Surgery, Vakif Gureba Educational Hospital, Istanbul, Turkey.
Eur Surg Res. 2009;42(2):71-7. doi: 10.1159/000171070. Epub 2008 Nov 13.
In this experimental study, we aimed to examine the protective effect of molsidomine (MS), a nitric oxide (NO) donor, against ischemia-reperfusion (I-R) injury in a rat skeletal muscle model.
Ischemia was achieved by application of an elastic rubber band as high as possible on the left thigh of the rats. Group 1: the control group received a sham operation. Group 2: the I-R group received I-R injury to the left hind limbs. Group 3: the I-R/MS group underwent the same model of I-R injury and received MS. Group 4: the I-R/L-NAME (N-omega-nitro-L-arginine-methyl ester) group underwent the same model of I-R injury and received L-NAME, an inhibitor of NO synthase.
In groups 2 and 4, malondialdehyde increased significantly when compared to groups 1 and 3. Superoxide dismutase, catalase and glutathione peroxidase increased significantly in group 3 compared to groups 2 and 4. The NO levels were significantly elevated in group 3 compared to groups 2 and 4. In addition, the histopathological score was considerably lower in group 3 than in group 4. The number of necrotic muscle fibers and infiltration of neutrophils were significantly reduced in the MS-treated group.
These findings suggest that MS can exert a protective effect against skeletal muscle injury caused by I-R in the rats.
在本实验研究中，我们旨在研究一氧化氮（NO）供体吗多明（MS）对大鼠骨骼肌模型缺血再灌注（I-R）损伤的保护作用。
通过在大鼠左大腿尽可能高位处扎上弹性橡皮筋来造成缺血。第1组：对照组接受假手术。第2组：I-R组对左后肢进行I-R损伤。第3组：I-R/MS组采用相同的I-R损伤模型并给予MS。第4组：I-R/L-NAME（N-ω-硝基-L-精氨酸甲酯）组采用相同的I-R损伤模型并给予L-NAME，一种一氧化氮合酶抑制剂。
与第1组和第3组相比，第2组和第4组丙二醛显著增加。与第2组和第4组相比，第3组超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶显著增加。与第2组和第4组相比，第3组一氧化氮水平显著升高。此外，第3组组织病理学评分明显低于第4组。MS治疗组坏死肌纤维数量和中性粒细胞浸润显著减少。
这些发现表明，MS对大鼠I-R所致骨骼肌损伤具有保护作用。
