Department of Cardiovascular Surgery, School of Medicine, Cumhuriyet University, Sivas, Turkey.
Biol Trace Elem Res. 2010 Nov;137(2):206-15. doi: 10.1007/s12011-009-8568-6. Epub 2009 Nov 24.
The present study investigated the protective effect of zinc aspartate, in connection with reactive oxygen species and nitric oxide, on long-term ischemia-reperfusion injury (IRI) in rat skeletal muscle. Following ketamine anesthesia, 24 rats were randomly assigned to four groups: groups 1 and 2, each without tourniquet application, received no drug and zinc, respectively; groups 3 and 4, each subjected to tourniquet-induced IRI (3 + 24 h), received no drug and zinc, respectively. IRI was achieved by the application of an elastic rubber band in the left hind limb of the anesthetized rats. Gastrocnemius muscle samples were obtained for biochemical measurements. Malondialdehyde levels were lower in group 2 and higher in group 3 than those seen in group 1. However, zinc aspartate (group 4) totally reversed malondialdehyde levels to control levels. Superoxide dismutase activity was increased in group 2 compared with group 1; however, there was no difference between groups 1 and 3, and Zn injection (group 4) increased superoxide dismutase activity. While catalase values were similar in groups 1 and 2, significant increments were observed in 3 and 4. A similar enhancement in glutathione levels were observed in groups 2 and 4 compared with group 1. Nitric oxide levels were lower in group 2 than 1, and no difference between groups 1 and 3 was demonstrated. In conclusion, zinc seems to be an effective treatment option against IRI.
本研究探讨了天门冬氨酸锌在与活性氧和一氧化氮相关联的情况下，对大鼠骨骼肌长期缺血再灌注损伤（IRI）的保护作用。在氯胺酮麻醉后，24 只大鼠被随机分为四组：第 1 组和第 2 组均未使用止血带，分别接受无药物和锌治疗；第 3 组和第 4 组（均应用止血带导致 IRI，即 3+24 小时），分别接受无药物和锌治疗。在麻醉大鼠的左后肢应用弹性橡胶带以实现 IRI。对腓肠肌样本进行生化测量。与第 1 组相比，第 2 组的丙二醛水平较低，第 3 组的丙二醛水平较高。然而，天门冬氨酸锌（第 4 组）将丙二醛水平完全逆转至对照水平。与第 1 组相比，第 2 组的超氧化物歧化酶活性增加；然而，第 1 组和第 3 组之间没有差异，并且锌注射（第 4 组）增加了超氧化物歧化酶活性。第 1 组和第 2 组的过氧化氢酶值相似，第 3 组和第 4 组显著增加。与第 1 组相比，第 2 组和第 4 组的谷胱甘肽水平均有类似的增加。第 2 组的一氧化氮水平低于第 1 组，第 1 组和第 3 组之间没有差异。总之，锌似乎是一种有效的 IRI 治疗选择。
