含有多孔聚合物囊泡作为级联反应纳米反应容器的酶。
Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions.
作者信息
Kuiper Suzanne M, Nallani Madhavan, Vriezema Dennis M, Cornelissen Jeroen J L M, van Hest Jan C M, Nolte Roeland J M, Rowan Alan E
机构信息
Molecular Materials, Institute for Molecules and Materials, University of Nijmegen, Toernooiveld 1, Nijmegen, Gelderland, Netherlands 6500 GL.
出版信息
Org Biomol Chem. 2008 Dec 7;6(23):4315-8. doi: 10.1039/b811196k. Epub 2008 Oct 15.
Polystyrene(40)-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)(50) (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.
聚苯乙烯（40）-b-聚（异氰基丙氨酸（2-噻吩-3-基-乙基）酰胺）（50）（PS-PIAT）聚合物囊泡具有独特的性质，其孔隙足够大，能使小（有机）底物扩散，同时将诸如酶等大分子保留在内部。在此，我们报道了葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）在PS-PIAT聚合物囊泡中的包封与保护，以及这些功能化纳米反应器在级联反应中的成功应用。所展示的概念可进一步应用于其他酶促级联反应、生物-有机混合系统和生物传感装置。
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