Hwang Junmo, Kim Hyung-Soo, Seok Ji-Woong, Kim Jun-Dae, Koun Soonil, Park So-Young, Lee Jinseok, Kim Hyang Sook, Kim Hyung-Seok, Kim Kil Soo, Chang Kyu-Tae, Ryoo Zae Young, Wang San Ming, Huh Tae-Lin, Lee Sanggyu
School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
Mol Genet Genomics. 2009 Jan;281(1):77-85. doi: 10.1007/s00438-008-0395-5. Epub 2008 Nov 13.
Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mib ( ta52b ) mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib ( ta52b ) mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib ( ta52b ) mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib ( ta52b ) mutant as compared with the wild-type. Interestingly, it was suggested that the mib ( ta52b ) mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.
Mind bomb（Mib）通过促进Notch配体的内吞作用来促进Notch信号通路。斑马鱼mib（ta52b）突变体在其泛素连接酶活性方面存在缺陷，而该活性对于抑制神经发生是必需的，这导致了神经元增生。mib（ta52b）突变体中一些受调控的基因已得到充分证实，然而，关于转录组图谱的报道相对较少。为了鉴定在mib（ta52b）突变体中差异表达的基因，使用基因表达序列分析进行了全基因组分析。鉴定出335个转录本，其在mib（ta52b）突变体中的表达与野生型相比有显著改变。有趣的是，提示mib（ta52b）突变可能不仅影响神经发生，还影响中胚层发育。这些结果为Notch信号通路提供了新的见解。
