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在斑马鱼视网膜发育过程中，miR-216a调控一种新型Notch信号通路组分snx5。

miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development.

作者信息

Olena Abigail F, Rao Mahesh B, Thatcher Elizabeth J, Wu Shu-Yu, Patton James G

机构信息

Department of Biological Sciences, Vanderbilt University, Nashville, TN.

出版信息

Dev Biol. 2015 Apr 1;400(1):72-81. doi: 10.1016/j.ydbio.2015.01.016. Epub 2015 Jan 31.

DOI:10.1016/j.ydbio.2015.01.016
PMID:25645681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4361363/
Abstract

Precise regulation of Notch signaling is essential for normal vertebrate development. Mind bomb (Mib) is a ubiquitin ligase that is required for activation of Notch by Notch׳s ligand, Delta. Sorting Nexin 5 (SNX5) co-localizes with Mib and Delta complexes and has been shown to directly bind to Mib. We show that microRNA-216a (miR-216a) is expressed in the retina during early development and regulates snx5 to precisely regulate Notch signaling. miR-216a and snx5 have complementary expression patterns. Knocking down miR-216a and/or overexpression of snx5 resulted in increased Notch activation. Conversely, knocking down snx5 and/or miR-216a overexpression caused a decrease in Notch activation. We propose a model in which SNX5, precisely controlled by miR-216a, is a vital partner of Mib in promoting endocytosis of Delta and subsequent activation of Notch signaling.

摘要

Notch信号的精确调控对于正常脊椎动物发育至关重要。Mind bomb（Mib）是一种泛素连接酶，是Notch的配体Delta激活Notch所必需的。分选连接蛋白5（SNX5）与Mib和Delta复合物共定位，并且已被证明可直接与Mib结合。我们发现，微小RNA-216a（miR-216a）在早期发育过程中在视网膜中表达，并调节snx5以精确调控Notch信号。miR-216a和snx5具有互补的表达模式。敲低miR-216a和/或过表达snx5会导致Notch激活增加。相反，敲低snx5和/或过表达miR-216a会导致Notch激活减少。我们提出了一个模型，其中由miR-216a精确控制的SNX5是Mib在促进Delta内吞作用及随后激活Notch信号方面的重要伙伴。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/0196b02fa069/nihms-662614-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/8236a967eb84/nihms-662614-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/5edc2a11a054/nihms-662614-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/7b68fb985f49/nihms-662614-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/88cd8757e3b2/nihms-662614-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/8f50579b5e75/nihms-662614-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/a36ce8debe74/nihms-662614-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/0196b02fa069/nihms-662614-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/8236a967eb84/nihms-662614-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/5edc2a11a054/nihms-662614-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/7b68fb985f49/nihms-662614-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/88cd8757e3b2/nihms-662614-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/8f50579b5e75/nihms-662614-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/a36ce8debe74/nihms-662614-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/895a/4361363/0196b02fa069/nihms-662614-f0007.jpg

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