Krzymińska Sylwia, Kaznowski Adam, Chodysz Magdalena
Faculty of Biology, Department of Microbiology, A. Mickiewicz University, ul. Umultowska 89, 61-614 Poznań, Poland.
Curr Microbiol. 2009 Mar;58(3):252-7. doi: 10.1007/s00284-008-9316-4. Epub 2008 Nov 13.
Interactions of Aeromonas caviae, Aeromonas veronii biotype sobria, and Aeromonas hydrophila strains isolated from fecal specimens of humans with gastroenteritis on murine macrophages, J774 cells, were investigated. Analyses of cellular morphology and DNA fragmentation in phagocytes infected with these strains exhibited typical characteristic features of cells undergoing apoptosis. We observed the morphological changes, including condensation of nuclear chromatin, formation of apoptotic bodies and blebbing of cell membrane, and fragmentation of nuclear DNA into oligonucleosomal fragments. The lowest apoptotic index did not exceed 25%, whereas the highest reached 78% at 24 h and 96% at 48 h after infection. After incubation of J774 cells with cytotoxic enterotoxin isolated from A. veronii biotype sobria strain, we noted that the toxin was able to trigger cytotoxicity and apoptosis of macrophages. The results indicate that apoptosis could be one of the mechanisms contributing to the development of Aeromonas-associated diarrheal disease.
研究了从患有肠胃炎的人类粪便标本中分离出的豚鼠气单胞菌、维氏气单胞菌温和生物型和嗜水气单胞菌菌株与小鼠巨噬细胞J774细胞的相互作用。对感染这些菌株的吞噬细胞进行细胞形态学和DNA片段化分析，显示出细胞凋亡的典型特征。我们观察到了形态学变化，包括核染色质浓缩、凋亡小体形成、细胞膜起泡以及核DNA断裂成寡核小体片段。最低凋亡指数不超过25%，而在感染后24小时最高达到78%，48小时达到96%。用从维氏气单胞菌温和生物型菌株中分离出的细胞毒性肠毒素孵育J774细胞后，我们注意到该毒素能够引发巨噬细胞的细胞毒性和凋亡。结果表明，凋亡可能是气单胞菌相关腹泻病发展的机制之一。
