Department of Microbiology, Faculty of Biology A. Mickiewicz University, 61-614 Poznań, ul. Umultowska 89, Poznan, Poland.
Antonie Van Leeuwenhoek. 2012 Feb;101(2):243-51. doi: 10.1007/s10482-011-9627-5. Epub 2011 Aug 2.
In the study we examined the production of cytotonic and cytotoxic toxins and the presence of a type III secretion system (TTSS) in 64 Aeromonas spp. strains isolated from fecal specimens of patients with gastroenteritis. We observed that contact of the bacteria with host epithelial cells is a prerequisite for their cytotoxicity at 3 h incubation. Cell-contact cytotoxic activity of the strains was strongly associated with the presence of the TTSS. Culture supernatants of the strains induced low cytotoxicity effects at the same time of incubation. Cell-free supernatants of 61 (95%) isolates expressed cytotoxic activity which caused the destruction of HEp-2 cells at 24 h. Moreover, 44% strains were cytotonic towards CHO cells and 46% of strains invaded epithelial cells.
在这项研究中，我们检测了 64 株从肠胃炎患者粪便标本中分离出的气单胞菌属菌株产生细胞毒性和细胞毒性毒素的情况，以及它们是否存在 III 型分泌系统（TTSS）。我们发现，细菌与宿主上皮细胞的接触是其在孵育 3 小时时具有细胞毒性的前提条件。菌株的细胞接触细胞毒性活性与 TTSS 的存在密切相关。同时，菌株的培养上清液在相同的孵育时间内诱导出低细胞毒性效应。61 株（95%）分离株的无细胞上清液表达细胞毒性活性，在 24 小时时导致 HEp-2 细胞破坏。此外，44%的菌株对 CHO 细胞具有细胞毒性，46%的菌株能够侵袭上皮细胞。
